Plasmid

Part:BBa_K4929004:Design

Designed by: Wang Jingmin   Group: iGEM23_SubCat-HongKong   (2023-08-23)


pAO815-AocI-FAD


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 286
    Illegal NheI site found at 1233
    Illegal NotI site found at 8485
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 4114
    Illegal BamHI site found at 86
    Illegal BamHI site found at 7832
    Illegal BamHI site found at 8222
    Illegal BamHI site found at 10833
    Illegal XhoI site found at 7142
    Illegal XhoI site found at 7531
    Illegal XhoI site found at 7671
    Illegal XhoI site found at 7717
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3111
    Illegal NgoMIV site found at 6956
    Illegal NgoMIV site found at 7702
    Illegal NgoMIV site found at 8425
    Illegal NgoMIV site found at 8535
    Illegal NgoMIV site found at 8651
    Illegal NgoMIV site found at 9740
    Illegal AgeI site found at 52
    Illegal AgeI site found at 6550
    Illegal AgeI site found at 6565
    Illegal AgeI site found at 7083
    Illegal AgeI site found at 8507
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2573
    Illegal BsaI site found at 3421
    Illegal BsaI.rc site found at 5487
    Illegal BsaI.rc site found at 6869
    Illegal BsaI.rc site found at 9824
    Illegal SapI site found at 4404
    Illegal SapI site found at 8087


Design Notes

NO


Source

NO

References